| Field | Specification |
|---|---|
| Target | |
| Alternative names | Max dimerization protein 3; Max dimerizer 3; Class C basic helix-loop-helix protein 13; bHLHc13; Max-associated protein 3; Max-interacting transcriptional repressor MAD3; Myx; MXD3; MAD3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAD3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21M81 is a rabbit monoclonal antibody that recognizes max dimerization protein 3 (gene MXD3) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 23.5 kDa, and the supplier reports an observed band at about 28 kDa.
MXD3 (MAD3) is a member of the MXD family of bHLH-leucine zipper repressors that pair with MAX. The MXD3-MAX heterodimer binds E-box DNA and antagonizes MYC-driven transcription and cell transformation, making MXD3 a subject of research on the MYC/MAX/MXD network and cell proliferation.
| Target | Max dimerization protein 3 (gene MXD3; UniProt Q9BW11, human) |
|---|---|
| Clone | 21M81 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.5 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MAD3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places max dimerization protein 3 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
