| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1, which shares 56.5% amino acid (aa) sequence identity with mouse MAdCAM1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mucosal addressin cell adhesion molecule 1 (gene MADCAM1) in human samples and is validated for IHC. The predicted molecular weight is 40.2 kDa.
Mucosal addressin cell adhesion molecule 1 (MADCAM1) is an immunoglobulin-superfamily adhesion molecule expressed on high endothelial venules of gut-associated lymphoid tissue and intestinal venules. By binding integrin α4β7 and L-selectin it guides lymphocytes into Peyer's patches and the intestinal lamina propria, so it is central to studies of gut immune homing and inflammatory bowel disease.
| Target | Mucosal addressin cell adhesion molecule 1 (gene MADCAM1; UniProt Q13477, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 40.2 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|
Samples with confirmed staining (IHC): human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human MAdCAM1. Sequence identity with the mouse ortholog: 56.5%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mucosal addressin cell adhesion molecule 1 in the membrane. Tissue expression noted by UniProt (human): Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
