| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MADCAM1, which shares 46.2% amino acid (aa) sequence identity with mouse MADCAM1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets mucosal addressin cell adhesion molecule 1 (gene MADCAM1) in human and rat samples and is validated for Western blot. The predicted molecular weight is 40.2 kDa, and the supplier reports an observed band at about 50 kDa.
Mucosal addressin cell adhesion molecule 1 (MADCAM1) is an immunoglobulin-superfamily adhesion molecule expressed on high endothelial venules of gut-associated lymphoid tissue and intestinal venules. By binding integrin α4β7 and L-selectin it guides lymphocytes into Peyer's patches and the intestinal lamina propria, so it is central to studies of gut immune homing and inflammatory bowel disease.
| Target | Mucosal addressin cell adhesion molecule 1 (gene MADCAM1; UniProt Q13477, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.2 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): human MCF-7 cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human MADCAM1. Sequence identity with the mouse ortholog: 46.2%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places mucosal addressin cell adhesion molecule 1 in the membrane. Tissue expression noted by UniProt (human): Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
