| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MADCAM1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24M63 is a rabbit monoclonal antibody that recognizes mucosal addressin cell adhesion molecule 1 (gene MADCAM1) in human samples and is validated for Western blot. The predicted molecular weight is 40.2 kDa, and the supplier reports an observed band at about 60 kDa.
Mucosal addressin cell adhesion molecule 1 (MADCAM1) is an immunoglobulin-superfamily adhesion molecule expressed on high endothelial venules of gut-associated lymphoid tissue and intestinal venules. By binding integrin α4β7 and L-selectin it guides lymphocytes into Peyer's patches and the intestinal lamina propria, so it is central to studies of gut immune homing and inflammatory bowel disease.
| Target | Mucosal addressin cell adhesion molecule 1 (gene MADCAM1; UniProt Q13477, human) |
|---|---|
| Clone | 24M63 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.2 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HUVEC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MADCAM1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mucosal addressin cell adhesion molecule 1 in the membrane. Tissue expression noted by UniProt (human): Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
