| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Laminin subunit alpha-3;Epiligrin 170 kDa subunit;E170;Epiligrin subunit alpha;Kalinin subunit alpha;Laminin-5 subunit alpha;Laminin-6 subunit alpha;Laminin-7 subunit alpha;Nicein subunit alpha;LAMA3;LAMNA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human MADCAM1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MADCAM1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24M63; isotype IgG; reactivity: Human. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-MADCAM1 Rabbit Monoclonal Antibody catalog # M04227-2. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: MADCAM1 (Laminin subunit alpha-3).
- Antibody format: Monoclonal; clone 24M63; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MADCAM1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Reported cellular localization context: Secreted, extracellular space, extracellular matrix, basement membrane. Major component. Tissue expression notes (as provided): Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
Research relevance and current trends
- Research context keywords from the source record include: Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate MADCAM1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MADCAM1 expression by Western blot in cell or tissue lysates, Compare relative MADCAM1 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 40-60 kDa; calculated MW: 366649 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 40-60 kDa
- Cellular localization (provided): Secreted, extracellular space, extracellular matrix, basement membrane. Major component.
- Tissue details (provided): Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
