| Field | Specification |
|---|---|
| Target | |
| Alternative names | Melanoma-associated antigen 1; Antigen MZ2-E; Cancer/testis antigen 1.1; CT1.1; MAGE-1 antigen; MAGEA1; MAGE1; MAGE1A |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAGE 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23M90 is a rabbit monoclonal antibody that recognizes melanoma-associated antigen 1 (gene MAGEA1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 46 kDa.
MAGEA1 is the founding member of the MAGE family of cancer-testis antigens, normally expressed in testicular germ cells but reactivated in many tumors, including melanoma. It was the first human tumor antigen shown to be recognized by cytotoxic T cells and has been reported to repress transcription through HDAC1 recruitment, so it is studied in tumor immunology.
| Target | Melanoma-associated antigen 1 (gene MAGEA1; UniProt P43355, human) |
|---|---|
| Clone | 23M90 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.3 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): A375 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MAGE 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places melanoma-associated antigen 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
