| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mucosa-associated lymphoid tissue lymphoma translocation protein 1; MALT lymphoma-associated translocation; Paracaspase; MALT1; MLT |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MALT1 Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADDA-13 is a rabbit monoclonal antibody that recognizes mucosa-associated lymphoid tissue lymphoma translocation protein 1 (gene MALT1) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 92.3 kDa, and the supplier reports an observed band at about 92 kDa.
MALT1 is a paracaspase that, together with CARD11 (or CARD9/CARD14) and BCL10, forms the CBM signaling complex downstream of antigen and pattern-recognition receptors. It acts both as a scaffold that recruits TRAF6 to activate NF-κB and as a protease that cleaves negative regulators, so it is a key target of study in lymphocyte activation and lymphoma.
| Target | Mucosa-associated lymphoid tissue lymphoma translocation protein 1 (gene MALT1; UniProt Q9UDY8, human) |
|---|---|
| Clone | ADDA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.3 kDa |
| Observed MW | 92 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HepG2 cells, human U251 cells, human A549 cells, human PC-3 cells, human SIHA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MALT1 Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mucosa-associated lymphoid tissue lymphoma translocation protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
