| Field | Specification |
|---|---|
| Target | |
| Alternative names | Microtubule-associated proteins 1A/1B light chain 3A;Autophagy-related protein LC3 A;Autophagy-related ubiquitin-like modifier LC3 A;MAP1 light chain 3-like protein 1;MAP1A/MAP1B light chain 3 A;MAP1A/MAP1B LC3 A;Microtubule-associated protein 1 light chain 3 alpha;MAP1LC3A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAP1LC3A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOD-13 is a rabbit monoclonal antibody that recognizes microtubule-associated protein 1 light chain 3 alpha (gene MAP1LC3A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 14.3 kDa, and the supplier reports an observed band at about 18 kDa.
LC3A (MAP1LC3A) is a ubiquitin-like protein of the ATG8 family. During autophagy it is attached to phosphatidylethanolamine on the growing phagophore membrane, where it helps elongate the membrane and binds selective autophagy receptors; it is used, like LC3B, as a marker of autophagosomes.
| Target | Microtubule-associated protein 1 light chain 3 alpha (gene MAP1LC3A; UniProt Q9H492, human) |
|---|---|
| Clone | EOD-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.3 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human SH-SY5Y cells, human U251 cells, human SiHa cells, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human MAP1LC3A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places microtubule-associated protein 1 light chain 3 alpha in the endomembrane system and cytoplasm. Tissue expression noted by UniProt (human): Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiang Jiao et al. (2025) Single-cell RNA sequencing revealed cell landscape of tongue dorsal mucosa in rats with gastric intestinal metaplasia. Cell Death Discovery. 10.1038/s41420-025-02386-z
Li Zichao et al. (2019) RETRACTED ARTICLE: Endoplasmic reticulum stress triggers Xanthoangelol-induced protective autophagy via activation of JNK/c-Jun Axis in hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-018-1012-z
Chen Feng et al. (2024) Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability. Cardiovascular Toxicology. 10.1007/s12012-024-09865-2
Jiaxing Li et al. (2024) Phillygenin rescues impaired autophagy flux by modulating the PI3K/Akt/mToR signaling pathway in a rat model of severe acute pancreatitis. International Journal of Immunopathology and Pharmacology. 10.1177/03946320241309260
