| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase kinase kinase 4; MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4; MAP3K4; KIAA0213; MAPKKK4; MEKK4; MTK1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAP3K4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28M41 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase kinase kinase 4 (gene MAP3K4) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 181.7 kDa, and the supplier reports an observed band at about 182 kDa.
MEKK4 (MAP3K4) is a MAP kinase kinase kinase that activates the p38 and JNK stress pathways, but not ERK, by phosphorylating MKK4 and MKK6. It is activated by GADD45 proteins after cellular stress and is also important for neural tube development.
| Target | Mitogen-activated protein kinase kinase kinase 4 (gene MAP3K4; UniProt Q9Y6R4, human) |
|---|---|
| Clone | 28M41 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 181.7 kDa |
| Observed MW | 182 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human U251 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MAP3K4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitogen-activated protein kinase kinase kinase 4 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
