| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1; MAP3K7; TAK1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAP3K7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACHF-13 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase kinase kinase 7 (gene MAP3K7) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 67.2 kDa, and the supplier reports an observed band at about 75 kDa.
TAK1 (MAP3K7) is a MAP kinase kinase kinase that, with its binding partners TAB1, TAB2 and TAB3, links IL-1, TNF, TGF-β and Toll-like receptor signals to downstream kinases. It activates the IKK complex, leading to NF-κB activation, as well as the JNK and p38 pathways, making it a central node in innate immunity and inflammation research.
| Target | Mitogen-activated protein kinase kinase kinase 7 (gene MAP3K7; UniProt O43318, human) |
|---|---|
| Clone | ACHF-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 67.2 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human 293T cells, rat lung tissue, rat brain tissue, mouse lung tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Synthetic peptide based on the sequence of human MAP3K7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitogen-activated protein kinase kinase kinase 7 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chen Yang et al. (2023) The protease calpain2a limits innate immunity by targeting TRAF6 in teleost fish. Communications Biology. 10.1038/s42003-023-04711-7
Ya Chen et al. (2022) eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish. Journal of Biological Chemistry. 10.1016/j.jbc.2022.101730
Yatao Bao et al. (2023) sNASP Mutation Aggravates to the TLR4-Mediated Inflammation in SLE by TAK1 Pathway. Journal of Immunology Research. 10.1155/2023/4877700
Wenjing Dong et al. (2025) RNF34 negatively regulates innate immunity via ubiquitin-mediated TAK1 degradation in miiuy croaker (Miichthys miiuy). Fish & Shellfish Immunology. 10.1016/j.fsi.2025.110578
Kaiyuan Ji et al. (2017) MicroRNA 143-5p regulates alpaca melanocyte migration, proliferation and melanogenesis. Experimental Dermatology. 10.1111/exd.13480
