| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase kinase kinase 8;2.7.11.25;Cancer Osaka thyroid oncogene;Proto-oncogene c-Cot;Serine/threonine-protein kinase cot;Tumor progression locus 2;TPL-2;MAP3K8;COT, ESTF; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MAP3K8 recombinant protein (Position: D9-L193). Human MAP3K8 shares 89.7% and 90.3% amino acid (aa) sequence identity with mouse and rat MAP3K8, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitogen-activated protein kinase kinase kinase 8 (gene MAP3K8) in human and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 52.9 kDa, and the supplier reports an observed band at about 53 kDa.
MAP3K8, also known as TPL2 or COT, is a serine/threonine kinase that activates MEK1/2 and ERK in macrophages after Toll-like receptor and cytokine stimulation. It is essential for LPS-induced TNF production and also influences T-helper cell differentiation, so it is studied in inflammation, innate immunity and cancer.
| Target | Mitogen-activated protein kinase kinase kinase 8 (gene MAP3K8; UniProt P41279, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 52.9 kDa |
| Observed MW | 53 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human A431 cells, human A549 cells, human THP-1 cells, human Daudi cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat brain tissue, mouse brain tissue, human lung cancer tissue, human pancreatic cancer tissue, human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human MAP3K8 fragment (Asp9–Leu193), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 89.7% and 90.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places mitogen-activated protein kinase kinase kinase 8 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in several normal tissues and human tumor-derived cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
