| Field | Specification |
|---|---|
| Target | |
| Alternative names | MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAPKAP Kinase 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19M94 is a rabbit monoclonal antibody that recognizes MAP kinase-activated protein kinase 2 (gene MAPKAPK2) in human samples and is validated for Western blot. The predicted molecular weight is 45.6 kDa, and the supplier reports an observed band at about 46 kDa.
MK2 (MAPKAPK2) is a serine/threonine kinase that sits directly downstream of p38 MAP kinase. Once phosphorylated by p38, it acts on targets such as HSP27 and tristetraprolin, controlling the stability and translation of mRNAs for TNF and other inflammatory mediators. It is a common readout in studies of stress signaling, inflammation and the DNA damage response.
| Target | MAP kinase-activated protein kinase 2 (gene MAPKAPK2; UniProt P49137, human) |
|---|---|
| Clone | 19M94 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.6 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MAPKAP Kinase 2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places MAP kinase-activated protein kinase 2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in all tissues examined. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
