| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Carnitine O-palmitoyltransferase 2, mitochondrial;2.3.1.21;Carnitine palmitoyltransferase II;CPT II;CPT2;CPT1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human MARK2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MARK2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20M53; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-MARK2 Rabbit Monoclonal Antibody catalog # M02117-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: MARK2 (Carnitine O-palmitoyltransferase 2, mitochondrial).
- Antibody format: Monoclonal; clone 20M53; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MARK2 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Reported cellular localization context: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. Tissue expression notes (as provided): Expressed by the liver and secreted in plasma.
Research relevance and current trends
- Research context keywords from the source record include: Axonal Guidance Proteins,Cell Adhesion Proteins,ECM Proteins,Growth and Development,Neurogenesis,Neurology Process,Neuroscience.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate MARK2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MARK2 expression by Western blot in cell or tissue lysates, Quantify MARK2-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 78 kDa, 82 kDa; calculated MW: 73777 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 78 kDa, 82 kDa
- Cellular localization (provided): Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
- Tissue details (provided): Expressed by the liver and secreted in plasma.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
