| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b; Par-1b; Par1b; MARK2; EMK1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MARK2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20M53 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase MARK2 in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 87.9 kDa; the supplier reports observed bands at 78, 82 kDa.
MARK2 (also called PAR-1b) is a serine/threonine kinase that phosphorylates microtubule-associated proteins such as tau, MAP2 and MAP4, loosening their grip on microtubules. As a member of the PAR polarity system, it helps establish cell polarity in epithelial cells and neurons. It is studied in cell polarity, neuronal development and tau phosphorylation.
| Target | Serine/threonine-protein kinase MARK2 (gene MARK2; UniProt Q7KZI7, human) |
|---|---|
| Clone | 20M53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 87.9 kDa |
| Observed MW | 78, 82 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MARK2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase MARK2 in the cell membrane, cytoplasm, lateral cell membrane and cell projection. Tissue expression noted by UniProt (human): High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
