| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Kinesin-like protein KIFC1;Kinesin-like protein 2;Kinesin-related protein HSET;KIFC1;HSET, KNSL2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human MARK3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MARK3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20M97; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-MARK3 Rabbit Monoclonal Antibody catalog # M05355-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: MARK3 (Kinesin-like protein KIFC1).
- Antibody format: Monoclonal; clone 20M97; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MARK3 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Minus end-ed microtubule-dependent motor required for bipolar spindle formation (PubMed:15843429). May contribute to movement of early endocytic vesicles (By similarity). Regulates cilium formation and structure (By similarity). . Reported cellular localization context: Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Early endosome . Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. . Tissue expression notes (as provided): Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
Research relevance and current trends
- Research context keywords from the source record include: Chromatin Modifying Enzymes,DNA/Nucleotides,DNA/RNA,Epigenetics and Nuclear Signaling,Methylation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate MARK3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MARK3 expression by Western blot in cell or tissue lysates, Localize MARK3 by immunofluorescence/immunocytochemistry in cultured cells, Quantify MARK3-positive cells by flow cytometry in single-cell suspensions, Enrich MARK3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 86 kDa; calculated MW: 73748 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 86 kDa
- Cellular localization (provided): Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Early endosome . Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. .
- Tissue details (provided): Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
