| Field | Specification |
|---|---|
| Target | |
| Alternative names | Methionine--tRNA ligase, cytoplasmic; Methionyl-tRNA synthetase; MetRS; MARS1; MARS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MARS1 recombinant protein (Position: R25-E887). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets methionine--tRNA ligase, cytoplasmic (gene MARS1) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry, immunoprecipitation and ELISA. The predicted molecular weight is 101.1 kDa, and the supplier reports an observed band at about 101 kDa.
MARS1 is the cytoplasmic methionyl-tRNA synthetase, which attaches methionine to its tRNA, including the initiator tRNA that starts protein synthesis. It is part of the multi-tRNA synthetase complex in the cytosol. Because it is essential for translation, it is studied in protein synthesis, cell growth and inherited disorders affecting the lung and nervous system.
| Target | Methionine--tRNA ligase, cytoplasmic (gene MARS1; UniProt P56192, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 101.1 kDa |
| Observed MW | 101 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL/ml (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL/ml |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human A549 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue, human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): human prostate cancer tissue.
Samples with a confirmed result (IP): A549 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human MARS1 fragment (Arg25–Glu887), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places methionine--tRNA ligase, cytoplasmic in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
