| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mannan-binding lectin serine protease 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2; MASP2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MASP2, which shares 88% and 76% amino acid (aa) sequence identity with mouse and rat MASP2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mannan-binding lectin serine protease 2 (gene MASP2) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 75.7 kDa, and the supplier reports an observed band at about 76 kDa.
MASP2 is the serine protease that drives the lectin pathway of complement activation. Bound to mannose-binding lectin or ficolins, it becomes active when these molecules recognize carbohydrate patterns on microbes, and it then cleaves C4 and C2 to form the C3 convertase. It is studied in innate immunity, infection and complement-mediated tissue injury.
| Target | Mannan-binding lectin serine protease 2 (gene MASP2; UniProt O00187, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 75.7 kDa |
| Observed MW | 76 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A431 cells, HepG2 cells.
Immunogen
Synthetic peptide from the C-terminal region of human MASP2. Sequence identity with the mouse and rat orthologs: 88% and 76%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes mannan-binding lectin serine protease 2 as secreted and also located in the cell surface. Tissue expression noted by UniProt (human): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
