| Field | Specification |
|---|---|
| Target | |
| Alternative names | Chymase; Alpha-chymase; Mast cell protease I; CMA1; CYH; CYM |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Mast Cell Chymase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18C83 is a rabbit monoclonal antibody that recognizes chymase (gene CMA1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 27.3 kDa, and the supplier reports an observed band at about 27 kDa.
Chymase (CMA1) is a chymotrypsin-like serine protease stored in mast cell granules and released on activation. It can convert angiotensin I to angiotensin II, process other vasoactive peptides and degrade extracellular matrix, so it is studied in mast cell biology, inflammation and cardiovascular remodeling.
| Target | Chymase (gene CMA1; UniProt P23946, human) |
|---|---|
| Clone | 18C83 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.3 kDa |
| Observed MW | 27 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human small intestine.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human mast Cell Chymase.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes chymase as secreted and also located in the cytoplasmic granule. Tissue expression noted by UniProt (human): Mast cells in lung, heart, skin and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
