| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tryptase alpha/beta-1;Tryptase-1;3.4.21.59;Tryptase I;Tryptase alpha-1;TPSAB1;TPS1, TPS2, TPSB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Mast Cell Tryptase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FHH-20 is a rabbit monoclonal antibody that recognizes tryptase alpha/beta-1 (gene TPSAB1) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 30.5 kDa, and the supplier reports an observed band at about 30 kDa.
Tryptase alpha/beta-1 (TPSAB1) encodes tryptase, the most abundant serine protease stored in mast cell granules and released when mast cells degranulate. Tryptase cleaves extracellular substrates such as fibronectin and may contribute to innate immunity. Serum tryptase is a standard marker of mast cell activation and burden in allergy and mastocytosis research.
| Target | Tryptase alpha/beta-1 (gene TPSAB1; UniProt Q15661, human) |
|---|---|
| Clone | FHH-20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.5 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human tonsil.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human mast Cell Tryptase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes tryptase alpha/beta-1 as a secreted protein. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
