| Field | Specification |
|---|---|
| Target | |
| Alternative names | S-adenosylmethionine synthase isoform type-2; AdoMet synthase 2; Methionine adenosyltransferase 2; MAT 2; Methionine adenosyltransferase II; MAT-II; MAT2A; AMS2; MATA2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MAT2A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25M89 is a rabbit monoclonal antibody that recognizes S-adenosylmethionine synthase isoform type-2 (gene MAT2A) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 43.7 kDa, and the supplier reports an observed band at about 48 kDa.
MAT2A is the catalytic subunit of methionine adenosyltransferase II, the enzyme that produces S-adenosylmethionine (SAMe) from methionine and ATP in most tissues outside the adult liver. SAMe supplies methyl groups for DNA, RNA, histone and protein methylation, and MAT2A is studied in epigenetics, cancer metabolism and methionine dependence of tumor cells.
| Target | S-adenosylmethionine synthase isoform type-2 (gene MAT2A; UniProt P31153, human) |
|---|---|
| Clone | 25M89 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.7 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human MAT2A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Detected in kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
