| Field | Specification |
|---|---|
| Target | |
| Alternative names | Multidrug and toxin extrusion protein 1; MATE-1; hMATE-1; Solute carrier family 47 member 1; SLC47A1; MATE1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MATE-1/SLC47A1 recombinant protein (Position: M1-D508). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets multidrug and toxin extrusion protein 1 (gene SLC47A1) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 61.9 kDa, and the supplier reports an observed band at about 32 kDa.
MATE1 (SLC47A1) is a proton/organic cation antiporter that pumps cationic compounds out of cells. Expressed mainly in kidney and liver, it helps excrete endogenous metabolites such as creatinine and thiamine, as well as many drugs and toxins, into urine and bile, making it a key transporter in drug disposition and drug–drug interaction research.
| Target | Multidrug and toxin extrusion protein 1 (gene SLC47A1; UniProt Q96FL8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 61.9 kDa |
| Observed MW | 32 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human MATE-1/SLC47A1 fragment (Met1–Asp508), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places multidrug and toxin extrusion protein 1 in the cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
