| Field | Specification |
|---|---|
| Target | |
| Alternative names | Matrin-3; MATR3; KIAA0723 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Matrin 3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18M47 is a rabbit monoclonal antibody that recognizes matrin-3 (gene MATR3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 94.6 kDa, and the supplier reports an observed band at about 125 kDa.
Matrin-3 (MATR3) is an RNA- and DNA-binding protein of the nuclear matrix. It participates in retaining defective RNAs in the nucleus together with the SFPQ-NONO complex, binds m6A-modified mRNAs, and contributes to innate immune signaling against DNA viruses. Mutations in MATR3 are associated with ALS and distal myopathy, so it is studied in RNA metabolism and neuromuscular disease.
| Target | Matrin-3 (gene MATR3; UniProt P43243, human) |
|---|---|
| Clone | 18M47 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 94.6 kDa |
| Observed MW | 125 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:150 |
Samples with a confirmed band (WB): human MCF-7 cells, human SH-SY5Y cells, human RT4 cells, human HepG2 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human lung cancer tissue, human esophageal cancer tissue, human rectal cancer tissue, human ovarian cancer tissue, human tonsil tissue, human liver cancer tissue, human pancreas cancer tissue, human placenta tissue, human stomach cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human matrin 3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places matrin-3 in the nucleus matrix. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
