| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial antiviral-signaling protein;MAVS;CARD adapter inducing interferon beta;Cardif;Interferon beta promoter stimulator protein 1;IPS-1;Putative NF-kappa-B-activating protein 031N;Virus-induced-signaling adapter;VISA;MAVS;IPS1, KIAA1271, VISA; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at N-terminus of human MAVS. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets mitochondrial antiviral-signaling protein (gene MAVS) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 56.5 kDa, and the supplier reports an observed band at about 75 kDa.
MAVS is an adaptor protein on the outer mitochondrial membrane that relays signals from the cytosolic RNA sensors RIG-I and MDA5. When these sensors detect viral RNA, MAVS forms large aggregates that activate TBK1 and IKK, leading to IRF3 and NF-κB activation and type I interferon production. It is a central readout in antiviral innate immunity research.
| Target | Mitochondrial antiviral-signaling protein (gene MAVS; UniProt Q7Z434, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 56.5 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Immunogen
Synthetic peptide based on the sequence of at N-terminus of human MAVS.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitochondrial antiviral-signaling protein in the mitochondrion outer membrane, mitochondrion and peroxisome. Tissue expression noted by UniProt (human): Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
