| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial antiviral-signaling protein;MAVS;CARD adapter inducing interferon beta;Cardif;Interferon beta promoter stimulator protein 1;IPS-1;Putative NF-kappa-B-activating protein 031N;Virus-induced-signaling adapter;VISA;MAVS;IPS1, KIAA1271, VISA; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Mavs recombinant protein (Position: M1-Q77). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets mitochondrial antiviral-signaling protein (gene Mavs) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 53.4 kDa, and the supplier reports an observed band at about 57 kDa.
MAVS is an adaptor protein on the outer mitochondrial membrane that relays signals from the cytosolic RNA sensors RIG-I and MDA5. When these sensors detect viral RNA, MAVS forms large aggregates that activate TBK1 and IKK, leading to IRF3 and NF-κB activation and type I interferon production. It is a central readout in antiviral innate immunity research.
| Target | Mitochondrial antiviral-signaling protein (gene Mavs; UniProt Q8VCF0, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 53.4 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, rat heart tissue, rat L6 cells, mouse brain tissue, mouse HEPA1–6 cells, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): ANA-1 cells.
Immunogen
Recombinant mouse Mavs fragment (Met1–Gln77), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places mitochondrial antiviral-signaling protein in the mitochondrion outer membrane, mitochondrion and peroxisome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Pengfei Wang et al. (2023) EFHD2 cooperates with E3 ubiquitin ligase Smurf1 to facilitate virus infection by promoting the degradation of TRAF6 in teleost fish. Journal of Virology. 10.1128/jvi.01176-23
