| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein max; Class D basic helix-loop-helix protein 4; bHLHd4; Myc-associated factor X; MAX; BHLHD4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human MAX recombinant protein (Position: A30-R106). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets protein max in human, mouse and rat samples and is validated for ELISA, flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 18.3 kDa, and the supplier reports an observed band at about 18–22 kDa.
MAX is a basic helix-loop-helix leucine zipper transcription factor that forms heterodimers with MYC, MXD and MNT family proteins. MYC-MAX dimers bind E-box sequences and activate genes for growth and metabolism, while MXD-MAX dimers generally repress them. As the common partner of this network, MAX is studied in cell proliferation, differentiation and cancer.
| Target | Protein max (gene MAX; UniProt P61244, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 18.3 kDa |
| Observed MW | 18–22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells, human PC-3 cells, human Jurkat cells, rat PC-12 cells, rat C6 cells, mouse RAW264.7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human MAX fragment (Ala30–Arg106), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein max in the nucleus and cell projection. Tissue expression noted by UniProt (human): High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
