| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mannose-binding protein C; MBP-C; Mannan-binding protein; RA-reactive factor P28A subunit; RARF/P28A; Mbl2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MBL2 recombinant protein (Position: E21-I248). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mannose-binding protein C (gene MBL2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 26.1 kDa, and the supplier reports an observed band at about 26 kDa.
Mannose-binding lectin (MBL, encoded by MBL2) is a liver-derived serum lectin that recognizes sugar patterns on bacteria, viruses, fungi and parasites. Bound to its associated MASP proteases, it triggers the lectin pathway of complement and promotes opsonization. Common genetic variants cause low serum MBL, and it is studied in innate immunity and susceptibility to infection.
| Target | Mannose-binding protein C (gene MBL2; UniProt P11226, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.1 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human hepatocellular carcinoma tumor tissue (HCCT).
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human MBL2 fragment (Glu21–Ile248), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes mannose-binding protein C as secreted and also located in the cell surface. Tissue expression noted by UniProt (human): Plasma protein produced mainly in the liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
