| Field | Specification |
|---|---|
| Target | |
| Alternative names | Muscleblind-like protein 1; Triplet-expansion RNA-binding protein; MBNL1; EXP; KIAA0428; MBNL |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MBNL1 recombinant protein (Position: Q89-M388). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects muscleblind-like protein 1 (gene MBNL1) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 41.8 kDa, and the supplier reports an observed band at about 40 kDa.
MBNL1 (muscleblind-like 1) is an RNA-binding protein that controls alternative splicing, mRNA localization and stability. It promotes adult splicing patterns in muscle, heart and brain. In myotonic dystrophy, expanded CUG or CCUG repeat RNAs sequester MBNL1, causing reversion to fetal splicing, which makes it a key protein in myotonic dystrophy research.
| Target | Muscleblind-like protein 1 (gene MBNL1; UniProt Q9NR56, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 41.8 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, monkey COS-7 cells, human 293T cells, human HeLa cells, human MOLT-4 cells, rat heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human thyroid cancer tissue, human colorectal adenocarcinoma tissue, human laryngeal squamous cells carcinoma tissue, human liver cancer tissue, human ovarian serous adenocarcinoma tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with confirmed staining (ICC/IF): human colon cancer tissue.
Immunogen
Recombinant human MBNL1 fragment (Gln89–Met388), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places muscleblind-like protein 1 in the nucleus, cytoplasm and cytoplasmic granule. Tissue expression noted by UniProt (human): Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiuying Liang et al. (2021) Comparative Proteomic Analysis of tPVAT during Ang II Infusion. Biomedicines. 10.3390/biomedicines9121820
