| Field | Specification |
|---|---|
| Target | |
| Alternative names | Muscleblind-like protein 1; Triplet-expansion RNA-binding protein; MBNL1; EXP; KIAA0428; MBNL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MBNL1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23M85 is a rabbit monoclonal antibody that recognizes muscleblind-like protein 1 (gene MBNL1) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 41.8 kDa, and the supplier reports an observed band at about 40 kDa.
MBNL1 (muscleblind-like 1) is an RNA-binding protein that controls alternative splicing, mRNA localization and stability. It promotes adult splicing patterns in muscle, heart and brain. In myotonic dystrophy, expanded CUG or CCUG repeat RNAs sequester MBNL1, causing reversion to fetal splicing, which makes it a key protein in myotonic dystrophy research.
| Target | Muscleblind-like protein 1 (gene MBNL1; UniProt Q9NR56, human) |
|---|---|
| Clone | 23M85 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.8 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MBNL1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places muscleblind-like protein 1 in the nucleus, cytoplasm and cytoplasmic granule. Tissue expression noted by UniProt (human): Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
