| Field | Specification |
|---|---|
| Target | |
| Alternative names | Maltose-binding periplasmic protein;MBP;MMBP;Maltodextrin-binding protein;malE;b4034, JW3994; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BD-13 is a rabbit monoclonal antibody that recognizes maltose/maltodextrin-binding periplasmic protein (gene malE) in human, mouse and rat samples and is validated for Western blot.
In Escherichia coli, maltose-binding protein (MalE, MBP) is a periplasmic binding protein that captures maltose and maltodextrins and delivers them to the MalFGK2 ABC transporter for import. Besides its role in sugar uptake and chemotaxis, MBP is widely used in the lab as a solubility-enhancing fusion tag for recombinant protein expression and purification.
| Target | Maltose/maltodextrin-binding periplasmic protein (gene malE; UniProt P0AEX9, Escherichia coli) |
|---|---|
| Clone | BD-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Ecoli.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
A synthesized peptide.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places maltose/maltodextrin-binding periplasmic protein in the periplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bin Wang et al. (2008) Nogo-66 Promotes the Differentiation of Neural Progenitors into Astroglial Lineage Cells through mTOR-STAT3 Pathway. PLoS One. 10.1371/journal.pone.0001856
