| Field | Specification |
|---|---|
| Target | |
| Alternative names | MBTD1; MBT domain-containing protein 1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MBTD1 recombinant protein (Position: M1-D420). Human MBTD1 shares 97.6% amino acid (aa) sequence identity with mouse MBTD1. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets MBT domain-containing protein 1 (gene MBTD1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
MBTD1 is a chromatin reader that binds mono- and dimethylated histone H4 lysine 20 (H4K20me1/2) through its MBT domains. As part of the NuA4/TIP60 histone acetyltransferase complex, it helps recruit the complex to DNA double-strand breaks, where it competes with 53BP1 and favors repair by homologous recombination. It is studied in chromatin biology and DNA repair.
| Target | MBT domain-containing protein 1 (gene MBTD1; UniProt Q05BQ5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL (Human) |
Immunogen
Recombinant human MBTD1 fragment (Met1–Asp420), expressed in E. coli. Sequence identity with the mouse ortholog: 97.6%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places MBT domain-containing protein 1 in the nucleus and chromosome.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
