| Field | Specification |
|---|---|
| Target | |
| Alternative names | Melanocortin receptor 4; MC4-R; MC4R |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MC4 Receptor |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20M08 is a rabbit monoclonal antibody that recognizes melanocortin receptor 4 (gene MC4R) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 36.9 kDa, and the supplier reports an observed band at about 43 kDa.
The melanocortin 4 receptor (MC4R) is a hypothalamic G protein-coupled receptor activated by α-MSH and blocked by agouti-related peptide. It acts as a key node in the leptin-melanocortin pathway that controls appetite and energy expenditure. MC4R mutations are the most common single-gene cause of severe early-onset obesity, so the receptor is widely studied in obesity research.
| Target | Melanocortin receptor 4 (gene MC4R; UniProt P32245, human) |
|---|---|
| Clone | 20M08 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.9 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): MCF-7 cells, RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MC4 Receptor.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places melanocortin receptor 4 in the cell membrane and cell projection. Tissue expression noted by UniProt (human): Brain, placental, and gut tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
