| Field | Specification |
|---|---|
| Target | |
| Alternative names | Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK; KIF2C; KNSL6 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MCAK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22K69 is a rabbit monoclonal antibody that recognizes kinesin-like protein KIF2C in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 81.3 kDa, and the supplier reports an observed band at about 81 kDa.
MCAK (KIF2C) is a kinesin-13 microtubule depolymerase that removes tubulin from microtubule ends. It is concentrated at centromeres and kinetochores, where it corrects improper microtubule attachments and helps ensure accurate chromosome segregation during mitosis. MCAK is studied in mitotic fidelity and is often overexpressed in tumors.
| Target | Kinesin-like protein KIF2C (gene KIF2C; UniProt Q99661, human) |
|---|---|
| Clone | 22K69 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 81.3 kDa |
| Observed MW | 81 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human MCF-7 cells, human K562 cells, human U2OS cells, human HEL cells, human A431 cells, human Jurkat cells, human PC-3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MCAK.
Reactivity Notes
The supplier lists reactivity with human. UniProt places kinesin-like protein KIF2C in the cytoplasm, nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
