| Field | Specification |
|---|---|
| Target | |
| Alternative names | Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT;MCL1;BCL2L3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MCL1, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes induced myeloid leukemia cell differentiation protein Mcl-1 (gene MCL1) in human samples and is validated for Western blot. The predicted molecular weight is 37.3 kDa, and the supplier reports an observed band at about 37 kDa.
MCL-1 is an anti-apoptotic member of the BCL-2 family that sits on the mitochondrial outer membrane. It binds and neutralizes pro-apoptotic proteins such as BAK and BIM, preventing mitochondrial outer membrane permeabilization. MCL-1 turns over rapidly, and because many cancers depend on it for survival, it is studied as a cancer target and in cell death regulation.
| Target | Induced myeloid leukemia cell differentiation protein Mcl-1 (gene MCL1; UniProt Q07820, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.3 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): HeLa cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human MCL1. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human. UniProt places induced myeloid leukemia cell differentiation protein Mcl-1 in the membrane, cytoplasm, mitochondrion and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiurui Yan et al. (2019) Nrf2/Keap1/ARE Signaling Mediated an Antioxidative Protection of Human Placental Mesenchymal Stem Cells of Fetal Origin in Alveolar Epithelial Cells. Oxidative Medicine and Cellular Longevity. 10.1155/2019/2654910
Weiqi Wu et al. (2021) Estrogen receptor-α36 is involved in diallyl sulfide-induced inhibition of malignant growth of HepG2 and Huh7 hepatocellular carcinoma cells. Environmental Toxicology. 10.1002/tox.23396
