| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA replication licensing factor MCM2;3.6.4.12;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;MCM2;BM28, CCNL1, CDCL1, KIAA0030; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MCM2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BCA-13 is a rabbit monoclonal antibody that recognizes DNA replication licensing factor MCM2 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 101.9 kDa, and the supplier reports an observed band at about 125 kDa.
MCM2 is a subunit of the MCM2-7 complex, the ring-shaped replicative helicase that is loaded onto origins of DNA replication and unwinds DNA during S phase. MCM2 also binds histones and helps handle them at the replication fork. Because MCM proteins are present in proliferating but not resting cells, MCM2 is widely used as a proliferation marker.
| Target | DNA replication licensing factor MCM2 (gene MCM2; UniProt P49736, human) |
|---|---|
| Clone | BCA-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 101.9 kDa |
| Observed MW | 125 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human A431 cells, human HEL cells, human HepG2 cells, rat thymus tissue, rat RH35 cells, mouse thymus tissue, mouse HEPA1–6 whole cellIHC: human bladder cancer tissue, human lung cancer tissue, human liver cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human breast cancer tissue, human tonsil tissue, human renal cancer tissue, human cervical cancer tissue, human ovarian cancer tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human MCM2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA replication licensing factor MCM2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Rong Yan et al. (2024) Multi-omics reveals the role of MCM2 and hnRNP K phosphorylation in mouse renal aging through genomic instability. Experimental Cell Research. 10.1016/j.yexcr.2024.114115
