| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA replication licensing factor MCM2;3.6.4.12;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;MCM2;BM28, CCNL1, CDCL1, KIAA0030; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MCM2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28M60 is a rabbit monoclonal antibody that recognizes DNA replication licensing factor MCM2 in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 101.9 kDa, and the supplier reports an observed band at about 125 kDa.
MCM2 is a subunit of the MCM2-7 complex, the ring-shaped replicative helicase that is loaded onto origins of DNA replication and unwinds DNA during S phase. MCM2 also binds histones and helps handle them at the replication fork. Because MCM proteins are present in proliferating but not resting cells, MCM2 is widely used as a proliferation marker.
| Target | DNA replication licensing factor MCM2 (gene MCM2; UniProt P49736, human) |
|---|---|
| Clone | 28M60 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 101.9 kDa |
| Observed MW | 125 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MCM2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA replication licensing factor MCM2 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
