| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102; MCM3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MCM3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFCC-13 is a rabbit monoclonal antibody that recognizes DNA replication licensing factor MCM3 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 91.0 kDa, and the supplier reports an observed band at about 100 kDa.
MCM3 is one of the six subunits of the MCM2-7 replicative helicase, which is loaded onto replication origins in G1 as part of the pre-replication complex and unwinds DNA during S phase. Its regulation helps ensure that the genome is copied exactly once per cell cycle, and it is studied in DNA replication and as a proliferation marker.
| Target | DNA replication licensing factor MCM3 (gene MCM3; UniProt P25205, human) |
|---|---|
| Clone | AFCC-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 91.0 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human MCM3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA replication licensing factor MCM3 in the nucleus and chromosome.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
