| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Solute carrier family 2, facilitated glucose transporter member 2;Glucose transporter type 2, liver;GLUT-2;SLC2A2;GLUT2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human MCM4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-MCM4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24M40; isotype IgG; reactivity: Human,Mouse. Reported application contexts include Flow, IP, IHC, WB (as provided in the source record). Boster Bio Anti-MCM4 Rabbit Monoclonal Antibody catalog # M02301. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: MCM4 (Solute carrier family 2, facilitated glucose transporter member 2).
- Antibody format: Monoclonal; clone 24M40; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
MCM4 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Facilitative glucose transporter. This isoform likely mediates the biional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na (+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Reported cellular localization context: Membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Liver, insulin-producing beta cell, small intestine and kidney.
Research relevance and current trends
- Research context keywords from the source record include: Adaptive Immunity,Cancer,Cancer Susceptibility,Cell Type Markers,Epigenetics and Nuclear Signaling,Immunology,NK Cells,Oncoproteins/Suppressors,Platelets,T Cells,Transcription,Tumor Suppressors.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate MCM4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect MCM4 expression by Western blot in cell or tissue lysates, Detect MCM4 in FFPE tissue sections by immunohistochemistry, Quantify MCM4-positive cells by flow cytometry in single-cell suspensions, Enrich MCM4 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 97 kDa; calculated MW: 57490 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 97 kDa
- Cellular localization (provided): Membrane; Multi-pass membrane protein.
- Tissue details (provided): Liver, insulin-producing beta cell, small intestine and kidney.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
