| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3; MCM7; CDC47; MCM2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MCM7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21M53 is a rabbit monoclonal antibody that recognizes DNA replication licensing factor MCM7 in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 81.3 kDa, and the supplier reports an observed band at about 81 kDa.
MCM7 is a subunit of the MCM2-7 replicative helicase, loaded onto origins of replication and activated to unwind DNA in S phase. It is required for replication licensing and interacts with checkpoint proteins that respond to replication stress. MCM7 is frequently overexpressed in proliferating tumor cells and is studied in DNA replication and cancer.
| Target | DNA replication licensing factor MCM7 (gene MCM7; UniProt P33993, human) |
|---|---|
| Clone | 21M53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 81.3 kDa |
| Observed MW | 81 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human MCM7.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA replication licensing factor MCM7 in the nucleus and chromosome.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
