| Field | Specification |
|---|---|
| Target | |
| Alternative names | Monocarboxylate transporter 1; MCT 1; Solute carrier family 16 member 1; SLC16A1; MCT1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Monocarboxylic acid transporter 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20S76 is a rabbit monoclonal antibody that recognizes monocarboxylate transporter 1 (gene SLC16A1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 53.9 kDa, and the supplier reports an observed band at about 50 kDa.
Monocarboxylate transporter 1 (MCT1, SLC16A1) moves lactate, pyruvate and ketone bodies across the plasma membrane together with a proton. Because the direction of transport follows substrate and proton gradients, MCT1 supports both lactate export from glycolytic cells and lactate uptake as a fuel, and it is widely studied in tumor metabolism and exercise physiology.
| Target | Monocarboxylate transporter 1 (gene SLC16A1; UniProt P53985, human) |
|---|---|
| Clone | 20S76 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 53.9 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human A431 cells, human HepG2 cells, human 293T cells, human PC-3 cells, human SIHA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human cervical cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human monocarboxylic acid transporter 1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places monocarboxylate transporter 1 in the cell membrane, basolateral cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
