| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcium uniporter protein, mitochondrial; HsMCU; Coiled-coil domain-containing protein 109A; MCU; C10orf42; CCDC109A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MCU recombinant protein (Position: V51-D351). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets calcium uniporter protein, mitochondrial (gene MCU) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 39.9 kDa, and the supplier reports an observed band at about 34 kDa.
MCU is the pore-forming subunit of the mitochondrial calcium uniporter, the channel in the inner mitochondrial membrane that takes up Ca2+ into the matrix. Mitochondrial Ca2+ uptake stimulates energy production and shapes cytosolic calcium signals, but excess uptake can trigger cell death. MCU is studied in metabolism, cardiac and neuronal function and cell survival.
| Target | Calcium uniporter protein, mitochondrial (gene MCU; UniProt Q8NE86, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 39.9 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human PANC-1 cells, monkey COS-7 cells, human HL-60 cells, human HepG2 cells, human Jurkat cells, rat brain tissue, rat kidney tissue, rat heart tissue, rat PC-12 cells, mouse brain tissue, mouse kidney tissue, mouse heart tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue, human liver cancer tissue, human bladder cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human MCU fragment (Val51–Asp351), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places calcium uniporter protein, mitochondrial in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiong Ya-bing et al. (2024) Mitochondrial calcium uniporter promotes kidney aging in mice through inducing mitochondrial calcium-mediated renal tubular cell senescence. ACTA Pharmacologica Sinica. 10.1038/s41401-024-01298-5
Chutian Zhang et al. (2025) Congrong Shujing Granules ameliorates mitochondrial associated membranes to against MPP+-induced neurological damage in the cellular model of Parkinson’s disease. Frontiers in Pharmacology. 10.3389/fphar.2025.1509317
Yan-Zhu Chen et al. (2026) Granulosa Cell Dysfunction Is Associated with MCU-Mediated Mitochondrial Calcium Dyshomeostasis and Reduced AKT Phosphorylation in Porcine Cystic Follicles. Theriogenology. 10.1016/j.theriogenology.2026.117913
Junbo Hong et al. (2025) MCU-Mediated SIRT3 Inhibition Drives Oxidative Stress-Induced Cytoskeletal Disruption in Pancreatic Ductal Epithelium: A Mechanism of Hypertriglyceridemic Acute Pancreatitis. International Journal of General Medicine. 10.2147/IJGM.S542782
