| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitochondrial calcium uniporter regulator 1; MCU regulator 1; Coiled-coil domain-containing protein 90A, mitochondrial; MCUR1; C6orf79; CCDC90A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MCUR1, which shares 79.4% and 82.3% amino acid (aa) sequence identity with mouse and rat MCUR1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitochondrial calcium uniporter regulator 1 (gene MCUR1) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 39.7 kDa, and the supplier reports an observed band at about 40 kDa.
MCUR1 is an inner mitochondrial membrane protein that regulates the mitochondrial calcium uniporter. It interacts with the pore-forming subunit MCU and is required for efficient Ca2+ uptake into mitochondria, likely by supporting assembly of the uniporter complex. It is studied in mitochondrial calcium handling and bioenergetics.
| Target | Mitochondrial calcium uniporter regulator 1 (gene MCUR1; UniProt Q96AQ8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 39.7 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): human HeLa whole cellhuman MDA-MB-231 cells, human HL-60 cells, human MDA-MB-453 cells, human A431 cells, human Caco-2 cells, rat spleen tissue, mouse lung tissue, mouse Ana-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human oesophagus squama cancer tissue, human ovary cancer tissue, human lung cancer tissue, human placenta tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the middle region of human MCUR1. Sequence identity with the mouse and rat orthologs: 79.4% and 82.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitochondrial calcium uniporter regulator 1 in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jie Ping et al. (2025) A highland-adaptation variant near MCUR1 reduces its transcription and attenuates erythrogenesis in Tibetans. Cell Genomics. 10.1016/j.xgen.2025.100782
Han Wei-Min et al. (2023) NMDARs antagonist MK801 suppresses LPS-induced apoptosis and mitochondrial dysfunction by regulating subunits of NMDARs via the CaM/CaMKII/ERK pathway. Cell Death Discovery. 10.1038/s41420-023-01362-9
Na Lu et al. (2023) Modulation of Oxidized Low-density Lipoprotein-Affected Macrophage Efferocytosis by Mitochondrial Calcium Uniporter in a Murine Model. Immunology Letters. 10.1016/j.imlet.2023.09.003
