| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon-induced helicase C domain-containing protein 1; Clinically amyopathic dermatomyositis autoantigen 140 kDa; CADM-140 autoantigen; Helicase with 2 CARD domains; Helicard; Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116; IFIH1; MDA5; RH116 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MDA5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADDD-9 is a rabbit monoclonal antibody that recognizes interferon-induced helicase C domain-containing protein 1 (gene IFIH1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 116.7 kDa, and the supplier reports an observed band at about 117 kDa.
MDA5 (IFIH1) is a RIG-I-like receptor that senses long double-stranded RNA and other viral RNAs in the cytoplasm. Upon binding RNA it signals through the mitochondrial adaptor MAVS to activate IRF3, IRF7 and NF-κB, driving type I interferon production, and it is studied in antiviral immunity and interferon-related autoimmunity.
| Target | Interferon-induced helicase C domain-containing protein 1 (gene IFIH1; UniProt Q9BYX4, human) |
|---|---|
| Clone | ADDD-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 116.7 kDa |
| Observed MW | 117 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MDA5.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interferon-induced helicase C domain-containing protein 1 in the cytoplasm, nucleus and mitochondrion. Tissue expression noted by UniProt (human): Widely expressed, at a low level. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
