| Field | Specification |
|---|---|
| Target | |
| Alternative names | Malate dehydrogenase, cytoplasmic; Aromatic alpha-keto acid reductase; KAR; Cytosolic malate dehydrogenase; MDH1; MDHA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MDH1 recombinant protein (Position: E56-A334). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects malate dehydrogenase, cytoplasmic (gene MDH1) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 36.4 kDa, and the supplier reports an observed band at about 36 kDa.
MDH1 is the cytoplasmic malate dehydrogenase, which interconverts malate and oxaloacetate using NAD+/NADH. It is a key part of the malate-aspartate shuttle that moves reducing equivalents from the cytosol into mitochondria, and it also supports gluconeogenesis. MDH1 is studied in cellular energy metabolism and cancer metabolism.
| Target | Malate dehydrogenase, cytoplasmic (gene MDH1; UniProt P40925, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 36.4 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human HeLa cells, human HepG2 cells, human HL-60 cells, rat liver tissue, rat spleen tissue, mouse liver tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human MDH1 fragment (Glu56–Ala334), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places malate dehydrogenase, cytoplasmic in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuntai Wang et al. (2025) Identification and validation of pyroptosis-related genes in Alzheimer’s disease based on multi-transcriptome and machine learning. Frontiers in Aging Neuroscience. 10.3389/fnagi.2025.1568337
