| Field | Specification |
|---|---|
| Target | |
| Alternative names | NADP-dependent malic enzyme, mitochondrial; NADP-ME; Malic enzyme 3; ME3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ME3 recombinant protein (Position: R17-D585). Human ME3 shares 95.8% amino acid (aa) sequence identity with mouse ME3. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NADP-dependent malic enzyme, mitochondrial (gene ME3) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
ME3 is the mitochondrial NADP-dependent malic enzyme, which converts malate to pyruvate and CO2 while producing NADPH inside mitochondria. It contributes to the mitochondrial NADPH supply that supports biosynthesis and antioxidant defense, and it is studied in energy and redox metabolism.
| Target | NADP-dependent malic enzyme, mitochondrial (gene ME3; UniProt Q16798, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ME3 fragment (Arg17–Asp585), expressed in E. coli. Sequence identity with the mouse ortholog: 95.8%.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places NADP-dependent malic enzyme, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed predominantly in organs with a low-division rate.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
