| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mediator of RNA polymerase II transcription subunit 6; Activator-recruited cofactor 33 kDa component; ARC33; Mediator complex subunit 6; hMed6; Renal carcinoma antigen NY-REN-28; MED6; ARC33 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human MED6 recombinant protein (Position: F40-Q246). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mediator of RNA polymerase II transcription subunit 6 (gene MED6) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 28.4 kDa, and the supplier reports an observed band at about 34 kDa.
MED6 is a subunit of the head module of the Mediator complex, conserved from yeast to humans. The head module directly contacts RNA polymerase II and supports formation of the transcription preinitiation complex at promoters. MED6 is studied in the core mechanisms of RNA polymerase II transcription.
| Target | Mediator of RNA polymerase II transcription subunit 6 (gene MED6; UniProt O75586, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.4 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human Jurkat cells, human HepG2 cells, human PC-3 cells, human U2OS cells, human A549 cells, human MCF-7 cells, rat C6 whole cellmouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human MED6 fragment (Phe40–Gln246), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mediator of RNA polymerase II transcription subunit 6 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
