| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myocyte-specific enhancer factor 2C;MEF2C; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MEF2C |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18M97 is a rabbit monoclonal antibody that recognizes myocyte-specific enhancer factor 2C (gene MEF2C) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 51.2 kDa, and the supplier reports an observed band at about 55 kDa.
MEF2C is a MADS-box transcription factor of the MEF2 family with key roles in heart, skeletal muscle, brain, bone and immune cell development. It cooperates with other factors to switch on tissue-specific gene programs and is regulated by calcium-dependent signaling and class II HDACs. MEF2C haploinsufficiency causes a neurodevelopmental disorder, and it is widely studied in development.
| Target | Myocyte-specific enhancer factor 2C (gene MEF2C; UniProt Q06413, human) |
|---|---|
| Clone | 18M97 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.2 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MEF2C.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places myocyte-specific enhancer factor 2C in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in brain and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
