| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Meis1; MEIS1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MEIS1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACDO-13 is a rabbit monoclonal antibody that recognizes homeobox protein Meis1 in human, mouse and rat samples and is validated for Western blot, immunoprecipitation and flow cytometry. The predicted molecular weight is 43.0 kDa; the supplier reports observed bands at 45,53 kDa.
MEIS1 is a TALE-class homeodomain transcription factor that partners with PBX and HOX proteins to regulate target genes. It is essential for hematopoietic stem cell maintenance, megakaryocyte and vascular development, and heart development. Together with HOXA9, MEIS1 drives certain acute myeloid leukemias, especially MLL-rearranged forms, and it is studied in development and leukemia.
| Target | Homeobox protein Meis1 (gene MEIS1; UniProt O00470, human) |
|---|---|
| Clone | ACDO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.0 kDa |
| Observed MW | 45,53 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human K562 cells, mouse pancrease tissue, human SH-SY5Y cells, human HeLa cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MEIS1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places homeobox protein Meis1 in the nucleus. Tissue expression noted by UniProt (human): Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
