Anti-MEK3 MAP2K3 Rabbit Monoclonal Antibody

Overview

Anti-MEK3 MAP2K3 Rabbit Monoclonal Antibody is an antibody targeting MAP2K3. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HDB-13; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 150 kDa; calculated MW: 39318 MW.

Boster Bio Anti-MEK3 MAP2K3 Rabbit Monoclonal Antibody catalog # M02916-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: MAP2K3 — Dual specificity mitogen-activated protein kinase kinase 3
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HDB-13; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 150 kDa; Calculated: 39318 MW

Biological background

Protein function (datasheet): Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Cellular localization (datasheet): Cytoplasm. Associated with polysomes. .

Tissue details (datasheet): Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

Research relevance and current trends

• Commonly studied in contexts related to Cancer,Epigenetics and Nuclear Signaling,Immunology,Innate Immunity,MAPK Pathway,Protein Phosphorylation,Ser/Thr Kinases,Serine/Threonine Kinases,Signal Transduction,TLR Signaling,Transcription,Tyrosine Kinases.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.