| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity mitogen-activated protein kinase kinase 4;MAP kinase kinase 4;MAPKK 4;2.7.12.2;JNK-activating kinase 1;MAPK/ERK kinase 4;MEK 4;SAPK/ERK kinase 1;SEK1;Stress-activated protein kinase kinase 1;SAPK kinase 1;SAPKK-1;SAPKK1;c-Jun N-terminal kinase kinase 1;JNKK;MAP2K4;JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human MEK4, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets dual specificity mitogen-activated protein kinase kinase 4 (gene MAP2K4) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 44.3 kDa, and the supplier reports an observed band at about 44 kDa.
MKK4 (MAP2K4) is a dual-specificity kinase in the stress-activated MAPK pathways. Together with MKK7 it is one of the few kinases that directly phosphorylate and activate the JNK kinases, and it can also activate p38; it is studied in stress signaling, apoptosis and as a tumor suppressor candidate.
| Target | Dual specificity mitogen-activated protein kinase kinase 4 (gene MAP2K4; UniProt P45985, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 44.3 kDa |
| Observed MW | 44 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human MEK4. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dual specificity mitogen-activated protein kinase kinase 4 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Abundant expression is seen in the skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
