| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity mitogen-activated protein kinase kinase 5;MAP kinase kinase 5;MAPKK 5;2.7.12.2;MAPK/ERK kinase 5;MEK 5;MAP2K5;MEK5, MKK5, PRKMK5; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MEK5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HCE-13 is a rabbit monoclonal antibody that recognizes dual specificity mitogen-activated protein kinase kinase 5 (gene MAP2K5) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 50.1 kDa, and the supplier reports an observed band at about 49 kDa.
MEK5 (MAP2K5) is a dual-specificity kinase that serves as the specific upstream activator of ERK5 (MAPK7). Scaffolding a complex with MEKK2 or MEKK3 and ERK5, it drives a MAPK pathway important for cell survival under stress, cardiovascular development and angiogenesis.
| Target | Dual specificity mitogen-activated protein kinase kinase 5 (gene MAP2K5; UniProt Q13163, human) |
|---|---|
| Clone | HCE-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 50.1 kDa |
| Observed MW | 49 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human MEK5.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed in many adult tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
