| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 5;MAP kinase kinase 5;MAPKK 5;2.7.12.2;MAPK/ERK kinase 5;MEK 5;MAP2K5;MEK5, MKK5, PRKMK5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human MEK5 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-MEK5 Rabbit Monoclonal Antibody is an antibody targeting MAP2K5. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HCE-13; isotype: Rabbit IgG; reactivity: Human; observed MW: 55 kDa; calculated MW: 50112 MW.
Boster Bio Anti-MEK5 Rabbit Monoclonal Antibody catalog # M03980. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MAP2K5 — Dual specificity mitogen-activated protein kinase kinase 5
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HCE-13; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 55 kDa; Calculated: 50112 MW
Biological background
Protein function (datasheet): Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. .
Cellular localization (datasheet): Cytoplasm, cytoskeleton . Cell projection, lamellipodium . At the interface between the lamellipodial actin meshwork and the membrane. .
Tissue details (datasheet): Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cardiogenesis,Cardiovascular,MAPK Pathway,Protein Phosphorylation,Ser/Thr Kinases,Serine/Threonine Kinases,Signal Transduction,Transcription Factors/Regulators.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
