| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity mitogen-activated protein kinase kinase 6;MAP kinase kinase 6;MAPKK 6;2.7.12.2;MAPK/ERK kinase 6;MEK 6;Stress-activated protein kinase kinase 3;SAPK kinase 3;SAPKK-3;SAPKK3;MAP2K6;MEK6, MKK6, PRKMK6, SKK3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human MEK6, identical to the related mouse sequence and different from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets dual specificity mitogen-activated protein kinase kinase 6 (gene MAP2K6) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 37.5 kDa, and the supplier reports an observed band at about 22–24 kDa.
MKK6 (MAP2K6) is a dual-specificity kinase that, together with MKK3, phosphorylates and activates the p38 MAP kinases. It is switched on by pro-inflammatory cytokines and environmental stress and is studied in inflammation, stress-induced cell death and differentiation.
| Target | Dual specificity mitogen-activated protein kinase kinase 6 (gene MAP2K6; UniProt P52564, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.5 kDa |
| Observed MW | 22–24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat ovary tissue, rat spleen tissue, rat brain tissue, rat heart tissue, NIH3I3 cells, HeLa cells, JRUKAT cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human MEK6. The peptide sequence is conserved in mouse. It differs from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dual specificity mitogen-activated protein kinase kinase 6 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Isoform 2 is only expressed in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
