| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase kinase kinase 2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MAP3K2; MAPKKK2; MEKK2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human MEKK2/MAP3K2 recombinant protein (Position: M1-Q364). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes mitogen-activated protein kinase kinase kinase 2 (gene MAP3K2) in human and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 69.7 kDa, and the supplier reports an observed band at about 78 kDa.
MEKK2 (MAP3K2) is a MAP kinase kinase kinase that activates MEK5 and MKK7, thereby driving the ERK5 and JNK signaling pathways. It is studied in growth factor and immune receptor signaling and has also been linked to caveolae dynamics.
| Target | Mitogen-activated protein kinase kinase kinase 2 (gene MAP3K2; UniProt Q9Y2U5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 69.7 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HEK293 cells, human HeLa cells, human K562 cells, human HepG2 cells, human MCF-7 cells, human Jurkat cells, rat kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human MEKK2/MAP3K2 fragment (Met1–Gln364), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places mitogen-activated protein kinase kinase kinase 2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
